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The results of this study indicate a consistent, strong relationship between the ActiGraph vertical component and the Actical counts per minute, as indicated by the high day by day and overall squared correlations.
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Mitogenic stimulation was achieved by supplementing 15% FCS/100 ng/ml tetradecanoylphorbol acetate (TPA; Sigma) for 45 minutes or as indicated.
Initial velocities (v) were obtained by fitting the data points of the time series to the equation for exponential association and calculating the tangent at 2 minutes, as indicated in Materials and Methods.
Activation and degradation of proteins in the NF-κB signaling pathway were analyzed by Western blot after the application of DCS in the absence or presence of IL-1β for 15 or 30 minutes as indicated in Figure 1.
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Cells were treated with NRGβ1 for 0 to 30 minutes as indicated, and cell lysates were analyzed for HER3 expression and Akt phosphorylation.
When SPION were suspended in the media, nanoparticles formed agglomerates during the first minutes, as indicated by the distribution of hydrodynamic particles size diameter, which was caused by the content of salts in the media.
Figure 3 provides information about the process of the formation of LCCs in mixture A. The entrapment was accomplished within 15 minutes as indicated by DLS intensity of Cel at 10 nm, but the LCCs kept dimensionally growing after the accomplishment of entrapment until reaching equilibrium at 60 minutes.
To determine the role of SR-BI on the regulation of signaling pathways, both shCTL and shSRBI MDA-MB-231 and MCF7 cells were serum starved overnight and then incubated in media containing 10% FBS for 30 minutes or 100 μg/ml of HDL3 for 0, 5, 15, and 30 minutes, as indicated.
Statistical significance was assessed as indicated.
Test, publish, review and change as indicated.
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