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Cells of interest were selected by observing the flat Epon embedded cell monolayer (containing the gridded Bellco print) under the light microscope, and mounted on pre-polymerized Epon blocks for thin sectioning.
Cells of interest were selected by observing the flat Epon embedded cell monolayer under the light microscope, and mounted on pre-polymerized Epon blocks for thin sectioning.
Images of immunostained sections were captured in a blinded fashion using a light microscope and mounted camera (M165FC and DFC310FX; Leica, Newcastle upon Tyne, United Kingdom).
For force recording, a small volume of the myofibril suspension was transferred to a chamber (15 °C) filled with a relaxing solution (pCa 9.0) on an inverted microscope and mounted horizontally between two glass microtools.
The arteries were dissected and cleaned free of fat and connective tissue under a light microscope and mounted as ring preparations on a small vessel myograph [ 6] to measure isometric tension.
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The cover slides were placed on a microscope slide, with the cells facing the surface of the microscope slide and mounted with CrystalMount.
After washing with ice-cold PBS, the cells were placed on microscope slides and mounted with Vectashield Mounting Medium (Vector Laboratories, Burlington, ON).
Cells were then washed with PBS and re-suspended in deionized H2O and transferred onto on microscope slides and mounted in Probing Antifade medium (Invitrogen) containing DAPI.
The glass slips contained within the cassettes were removed, affixed onto regular glass microscope slides, and mounted in lactic acid with cotton blue.
Following dissection of secretory stage enamel, incisor teeth were glued to glass microscope slides and mounted in polypropylene tubes for CT scanning.
Ovaries were placed on a microscope slide and mounted with glycerol, a drop of SlowFade Gold antifade reagent (Invitrogen, Grand Island, NY, USA), stained with DAPI, and visualized with a Nikon TE-2000 usinvertedrted fluorescence microscopy (Nikon, Melville, NY, USA).
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