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The stained sections were viewed on a JEOL 100CX transmission electron microscope, and documented with a SIS (Soft Imaging Systems, Lakewood, CO) MegaView III digital side mount camera.
The morphology of PMN in the cell culture was monitored using an invert microscope and documented by digital imaging (Carl Zeiss, Jena, Germany).
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Cellular structures were evaluated 1, 2, and 3 weeks after seeding by light microscopy and documented using a Leitz Labovert (Leitz, Wetzlar, Germany) inverted microscope.
The CK+ cells were identified by two independent investigators under a BX60 fluorescent microscope (Olympus, Tokyo, Japan) and documented with a DP10 camera (Olympus).
The results were analyzed in a Zeiss Axioplan 2 epifluorescence microscope (Carl Zeiss Jena GmbH, Jena, Germany) and documented using a SD200 Spectracube system (ASI).
Tubes were viewed under an inverted transmission microscope (Zeiss Axiovert 200 M) and documented with a digital imaging system (Axiovision Software, Zeiss).
Histological slides were evaluated under an Olympus light microscope (BX45; Olympus, Hamburg, Germany) and documented with a digital camera (Camedia C7070; Olympus).
Sections were examined using a Zeiss AxioImager microscope equipped with epifluorescent optics and documented using a Hamamatsu ORCA ER camera or with a Zeiss confocal laser scanning system LSM 510meta.
The results were analysed and documented with the microscope AxioPlan 2 and an AxioCam camera (Zeiss) using the software AxioVision Rel.
In the next step cells were incubated with neutral red dye for 15 minutes, washed triple times in PBS, and observed and documented using inverted microscope and digital camera.
Serial sections were cut to a thickness of 5 7 μm and documented using a Zeiss microscope (Axiovert 40).
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