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Each placenta sample was tested for maternal DNA contamination and to confirm maternal identity by PCR amplification of 17 microsatellites in different autosomes and the amelogenin locus (data not shown).
These findings raise questions as to why microsatellites might be conserved in distant species, and why microsatellites in different genomic locations are maintained to different extents.
Moreover, the frequency and distribution of these microsatellites in different genomic regions were analyzed and an integrative database of tetranucleotide microsatellite markers was developed.
As highlighted in previous studies using the same microsatellites in different geographical areas [ 39- 41], all microsatellites were detected as being polymorphic in each of the SL of the southern African sub-region.
In this study, the entire genome of a monokaryotic V. volvacea strain (V23-1) has been screened to determine the distribution and density of microsatellites in different genomic regions.
More recently, Cavagnaro et al. [ 24] have analyzed microsatellites in different plants, and found that tri-nucleotide repeats are the most prevalent type of SSRs in Arabidopsis, soybean, rice, and sorghum, while tetra-nucleotide repeats are most prevalent in poplar and grapevine.
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This could be due to differences in polymorphism level of the microsatellites used in different studies (Hedrick 1999).
Thus, together with 24 microsatellites located in different BAC clones and used for screening the BAC library, a total of 86 BAC based markers have been anchored on the linkage map with at least one on each LG.
The observed differences of heterozygosity of the MHC and microsatellites in the different sexes and across age classes are thus more likely to reflect survival advantage of MHC DRB heterozygous males than an effect of population dynamics.
The frequency of occurrence of microsatellites had varied in different databases of Eucalyptus for example, 12.9% in NCBI database (Yasodha et al. [2008]) 13.3% in EUCAWOOD (Rengel et al. [2009]), 25.5% and 29% in FORESTs database (Rabello et al. [2005]; Ceresini et al. [2005]).
These microsatellites were characterized in different species of ungulates: IDVGA29, IDVGA8, CSSM39, CSSM41, CSSM43, BM1706, HUJ1177, BMC1009, BM848, BM757 in Bos taurus, OarFCB304 in Ovis aries and NVHRT48 in Rangifer tarandus [17].
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