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For most of this period irreversible electroporation (IRE) was used primarily for ablation of microorganisms and cells in vitro and studied only as an upper limit of electrical parameters for reversible tissue electroporation applications.
One of the most important goals of contemporary biology is to understand the elemental principles governing metabolic structure as a whole which underlie the common design of all microorganisms and cells.
The WFCC collects, authenticates, manages, and distributes cultured microorganisms and cells.
The WFCC pioneered the development of an international database for cultured microorganisms and cells, which led to the establishment of the WDCM.
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Authentication of cell lines was conducted by the Leibniz Institute DSMZ-German Coflection of Microorganisms and Cell Cultures.
MEC-1 and MEC-2 human leukemia cells were purchased from Leibniz Institute DSMZ-German Coflection of Microorganisms and Cell Cultures (DSMZ, Germany).
Metabolic engineering has as a goal the improvement of yield of desired products from microorganisms and cell lines.
Caco-2 and HT-29 (German collection of microorganisms and cell cultures, ACC 169 and ACC 299, respectively), and HCT-8 (ATCC CCL-244) were cultured in Quantum 286 epithelial medium (GE Healthcare).
Leibnitz Institute DSMZ, German Collection of Microorganisms and Cell Cultures.
Cell line was purchased from the Leibniz Institute DSMZ-German Coflection of Microorganisms and Cell Cultures: CCRF-CM (ACC 240).
The cell line was obtained from the Collection of Microorganisms and Cell Cultures of the Leibniz Institute DSMZ-German.
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