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These culture conditions were repeated for independent samples and cells were harvested to determine the transcriptional and translational responses of the cells to treatment with LC-uFFAs.
Ck/B/99 virus antigen positive cells were found in all tissues of the respiratory tract, but the numbers of positive samples and cells were minimal, especially in the upper respiratory tract (Figure 2).
Tissue samples and cells were fixed and embedded in paraffin.
Nuclear extracts of tissue samples and cells were used as inputs for DNA-protein binding purification.
Total RNA was extracted by miRNeasy kit (Qiagen) from both tissue samples and cells.
Unstained cell samples and cells stained with Annexin V or PI only were prepared for fluorescence compensation.
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The San Diego Zoo has its parallel Frozen Zoo, an archive of thousands of DNA samples and cell lines from a host of species.
Another specific and sensitive tool for diagnosing chikungunya infection is the one-step TaqMan RT-PCR assay that quantifies viral load in both clinical samples and cell culture.
Polymerase chain reaction assays have become widely used methods of confirming the presence of Mollicutes species in clinical samples and cell cultures.
As shown in Figure 1a and 1c, the edited substrates were amplified successfully in all tissue samples and cell lines.
We conducted analysis to identify genome-wide aUPD regions using data from 700 breast tumor samples and cell lines.
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