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We explored potential contributions of the following factors to explain lower culture conversion rates in Africa: 1) baseline severity of disease, 2) microbiological procedures, and 3) pharmacokinetic characteristics (published elsewhere, [11]).
JLD was involved in the microbiological procedures and data collection.
OK and I Sencan: Patient management, article conception and critical, extensive revision of article for important intellectual content, I Sahin: microbiological procedures and statically analysis.
The single central laboratory undertook the microbiological procedures and the input of data from the questionnaires in a purpose-structured database.
The investigators who performed microbiological procedures and those who selected control subjects were unaware of the vaccination histories of the subjects.
HV was the principal investigator, participated in the planning and execution of the work, including performing the on-site microbiological procedures, and analysis of data, and was the main responsible author.
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Blood culture isolates were identified using standard microbiological procedure and further confirmation was done using commercially available API strips [ 12].
Bacterial isolates were identified using standard microbiological procedures, as growth and colonial characteristics, Gram staining, cellular morphology, catalase and oxidase reactions, coagulase test (Slidex Staph Plus, bioMérieux, Marcy-l'Etoile, France), haemolysin production.
Eyelid margin and conjunctival swabs were collected and processed using standard microbiological procedures to identify bacterial isolates and their respective antimicrobial susceptibility patterns.
GP, EM and EN carried out the molecular studies; RR, JC and AC, obtained the gastric biopsy samples; SA and CR carried out the microbiological procedures; SV conceived of the study, and participated in its design and coordination, and drafted the manuscript.
Microbiological procedures for Brucella detection and identification have been controversial for years.
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