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Plastic wells were filled with liquid medium, inoculated with 300 μL 0.5 McFarland microbial suspension and incubated for 72 h at 30°C.
Cyclic operation is imposed by the periodic harvesting of a fraction of the microbial suspension and replenishment of the harvested volume with an equal volume of fresh medium.
Next, 500 μl of hydrocarbon was added to 5 ml of microbial suspension and vortexed for 2 min.
This instrument consisted of a 400 mL vessel with an electrical heater for thermostation, an agitation device used to ensure inoculum distribution and temperature homogeneity, a pressurization system, and ports for injecting the microbial suspension and for extraction of samples.
Previous studies have already verified the antifungal activity of vinegars [ 16], although MIC and MFC values are not expressed according to the microdilution technique, which is a low-cost and rapid method that provides reproducible results and requires small amounts of microbial suspension and culture media [ 14, 17, 18].
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The plastic wells were inoculated with 300 μL of 0.5 McFarland microbial suspensions and incubated for 24 h at 37°C.
Plastic wells were filled with a liquid medium, inoculated with 300 μL of 0.5 McFarland microbial suspensions and incubated for 72 h at 30°C.
The plastic wells were filled with sterile liquid culture medium, inoculated with 300 μL of 0.5 McFarland microbial suspensions and incubated for 72 h at 30°C.
In order to examine what may be the effect of various size classes of soil biota we compared inoculation with natural field soil sieved through a 1 mm mesh, a soil suspension also sieved through a 1 mm mesh, and a microbial suspension sieved through a 20 μm mesh.
The use of the test microbial suspension is to monitor the disinfection procedure and its performance is dependent on both the initial test microbial suspension population (N0) and the D-value [ 9, 10].
Following incubation, the microbial suspension was removed from the canal lumen and inoculated onto Saboraud Dextrose Agar to obtain the number of colony forming units.
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algal suspension and
bacterial suspension and
extract suspension and
challenge suspension and
microbial activity and
microbial world and
microbial aetiology and
microbial disease and
microbial diversity and
microbial identification and
microbial population and
microbial zoo and
microbial growth and
microbial contamination and
microbial composition and
microbial load and
microbial staining and
microbial adhesion and
microbial metabolism and
microbial sepsis and
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