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Thus, the glycan-related gene expression profiles are expressed as the ratio of the gene expression signal at each spot on the microarray relative to the reference RNA signal.

The analysis compared the expression levels of every single spot on the microarray relative to a reference sample, and then compared the relative expression levels between stressed and control birds, or between birds whose parents had received stress or control treatment.

For this analysis, we compared the percentage of genes identified by SAGE that were also identified by microarray, relative to the number of tags identified for the genes.

DAVID was used to investigate the representation of broad gene ontology (GO) categories (Level 1) on the equine cDNA microarray relative to the whole genome.

Figure 4a shows the position of the sequence queried on the CGI microarray relative to the region that was bisulfite sequenced, and the NKX2-3 gene sequence.

As displayed in Figure 4, the microarray and real-time PCR results agreed on the expression direction (either up-regulated or downregulated) of all genes but, as shown previously [ 44, 45], the changes were underestimated by the microarray relative to the real-time PCR results.

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Similar(53)

It is nevertheless possible that some of the false-positives in microarrays relative to MPSS could be caused by cross-hybridization due to suboptimal probe design.

The resulting mRNA pool is subsequently reverse transcribed into cDNA and analysed on microarrays, relative to the transcripts generated in the absence of the regulatory protein.

In the majority of cases the RTqPCR data confirmed, and correlated significantly with, the microarray data (Table 6) although expression ratios were generally underestimated by the microarrays relative to the RTqPCR (Additional file 6).

The maturity of gene expression microarrays relative to similar technologies designed to measure other molecular phenotypes on a genomic scale has meant that gene networks primarily are rendered as gene coexpression networks.

There were also a number of genes that can be regarded as "false-negatives" on the microarrays relative to MPSS, in the sense that they were represented on the microarrays and detected by MPSS but not detected as expressed by the microarrays.

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