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Values were calculated after processing microarray probe data using MAS5 [97], dCHIP [98] and RMA [99] algorithms implemented in the R package affy; RNA degradation plots and associated parameters [91].
The microarray probe data were retrieved from Affymetirx.
For example, one might replace microarray probe data with more sensitive qRT-PCR data.
In addition to the proteomics data, microarray probe data were collected for Y. pestis Pestoides F [ 26].
We retrieved GO http://www.geneontology.org/ annotations as assigned to the unigenes in the microarray probe data set and used Fisher's exact test as implemented by Blast2GO to check for enriched GO categories when the tests sets were compared to the total query [ 86].
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A total of 9,112 Ensembl ID genes could be retrieved from microarray probes data for RNA-seq comparisons.
To determine relative differences in gene length parameters, a custom python script was written that links microarray probe set data to gene structural information from the Ensembl database (PMID: 17148474) obtained through BioMart [60].
Next, we describe software called "GenePool," which directly analyzes SNP microarray probe intensity data and ranks SNPs by increased likelihood of being genetically associated with a trait or disorder.
MochiView uses an integrated local database to manage all of the data imported by the user, such as genome sequences and alignments, gene locations, microarray probe locations, expression data, ChIP data, and motif libraries.
All microarray probes and data discussed in this publication have been deposited in NCBI's Gene Expression Omnibus[ 57] and are accessible through GEO Series accession number GSE18347.
GC Robust Multi-array Average (GCRMA) background adjustment, quantile normalization and median-polish summarization on Affymetrix HGU133 plus 2.0 microarray probe-level data were performed using R 2.7.1 and Bioconductor 2.2.
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