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The Affymetrix GeneChip® platform (Affymetrix, Santa Clara, CA, USA) is a widely used microarray technology in which each gene on the microarray is represented by multiple 25-mer oligonucleotide probes.
However, because each gene on the BOTL-5 microarray is represented twice, individual spot features are not strictly independent observations.
Although the discrimination between molecules represents a great challenge, the main advantage of miRNA microarray is represented by the high specificity of the reaction, whereas the main disadvantage is represented by low sensitivity [ 21].
Each timepoint on the microarray is represented by a unique color, to indicate significant upregulation in the microarrays at the indicated timepoints (adjusted P<0.05 and log2 fold change>0.4).
Each individual gene transcript on the Affymetrix Rice and Arabidopsis ATH-1 GeneChip® microarray, is represented by a set of eleven, 25 bp 'probe-pairs' that make up a 'probe-set', and it is the average hybridisation intensity across this probe-set that is used to calculate expression levels for that gene.
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Because reverse transcription of bacterial RNA used random primers, and because each gene on the microarray was represented by seventeen probes, microarray experiments were nonetheless likely to capture most of the abundant RNAs.
Only peaks that fall in the regions covered by the Affymetrix mouse promoter microarrays are represented.
The expression profile of selected significantly regulated genes from microarrays was represented by heat maps using the R statistical software.
Today, state-of-the-art microarray technology is represented by high-density whole-genome tiling arrays.
The basal level is denoted by qk, and the stochastic noise due to model uncertainty and fluctuation of the microarray data is represented by nk(t).
The basal level is denoted by q k, and the stochastic noise due to model uncertainty and fluctuation of the microarray data is represented by n k t.
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