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The design of the microarray is described in detail elsewhere [ 6, 7] and a full description of the platform and data presented in this manuscript are accessible through the public GEO depositories (accession number GPL6155 and GSE10272).
S. coelicolor microarray containing duplicate spots representing 90% of the genes in the genome was used for hybridizations to compare RNA from Samples 1 and 2. The construction of the microarray is described on our website at. 10 μg of total RNA was used for each sample as starting material.
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Details of the construction and evaluation of the microarray were described previously [7].
A sequence-independent 'universal' microarray was described in [ 16].
Recently, a FMDV microarray was described to detect simultaneously the seven FMDV serotypes.
Standard procedures for miRNA microarray are described in Supplementary Materials and Methods.
The immunohistochemistry protocols and the method of constructing tissue microarray are described elsewhere (Zhu et al, 2008).
In this work an automated electrical readout system for low-cost glass-slide microarrays is described.
For Protocol A, sampling of the chemostat cultures, probe preparation and hybridization to the single-channel Affymetrix GeneChip YG S98 microarrays is described in Piper et al. [ 29].
It has been 10 years since tissue microarrays were described by Kononen et al. for the first time.
The Mt16KPlus microarrays are described at http://www.ebi.ac.uk/arrayexpress (accession number A-MEXP-138).
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