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At the beginning of our analysis, the grouping of the microarray hybridizations was still unknown to the data analysts.
The expression of genes selected from the results of the microarray hybridizations was verified using a qRT-PCR approach.
a responder sample in microarray analysis b non-responder sample in microarray analysis *: RNA used in the microarray hybridizations was not available for this test.
Normalization to correct for technical variation among individual microarray hybridizations was conducted using a two-step procedure described in detail elsewhere [ 15].
The quality of human promoter microarray hybridizations was validated by quantitative PCR, showing that most of the detected regions have similar modification patterns as found by promoter microarray profiling.
To determine the effects of GCRs on the patterns of gene expression, each pair of mutants and parental strains were grown in minimum liquid medium up to mid-log phase and a comparative transcriptomic analysis based on microarray hybridizations was conducted.
Similar(54)
Microarray hybridizations were performed in triplicates for each strain.
Microarray hybridizations were performed on the 22K Agilent platform.
The microarray hybridizations were designed as common reference experiments.
Behavioral data were recorded, whole hypothalami were collected, and microarray hybridizations were performed.
Microarray hybridizations were performed on Affymetrix MG-U74Av2 chips through the BCM Microarray Core Facility.
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