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SJW performed microarray hybridizations, analyzed the data and wrote the manuscript.
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YH performed microarray hybridization, analyzed part of microarray and cDNA library datasets.
FC designed the experiment, collected the samples, performed the microarray hybridization, analyzed the data and wrote the manuscript.
EB isolated RNA and developed the G. holbrooki cDNA library using the MINT cDNA synthesis and TRIMMER kits, isolated RNA from the tissue samples used for the microarray analysis, conducted the cRNA amplification and microarray hybridization, analyzed the microarray data, and conducted the qPCR analysis.
All in all, data from 6 separate microarray hybridizations were analyzed in this study.
performed amplifications and microarray hybridizations; W.E. analyzed morphology of blood cells and provided critical expertise; N.S.F.
Eleven genes representing different functional categories and range of gene expression values based on microarray hybridizations were analyzed using qPCR from cDNA derived from different time point samples.
Six genes representing a range of gene expression values based on microarray hybridizations were analyzed using qPCR from cDNA derived from different time point samples.
Seventeen genes representing different functional categories and the range gene expression values, based on microarray hybridizations were analyzed using qPCR from cDNA derived from stationary phase samples.
Aliquots of RNA transcripts used for microarray hybridizations were analyzed for A- and D-genome contributions to the transcriptome for AD1 and F1 samples (the validation design can be found in Additional file 1).
To identify p53 binding sites in these scenarios and p53's effect on the epigenetic state, we conducted chromatin immunoprecipitation coupled to microarray hybridization that analyzed 13,000 human gene promoters.
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