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Performance of this method was compared with the popular microarray analysis method Significant Analysis of Microarrays (SAM) [12].
Paired t-tests and paired significant microarray analysis method (SAM) were used to evaluate the influence of one particular variability factor (TNF, PL, RBC, and time).
These results confirmed the reliability of microarray analysis method in this study.
This issue is addressed in the microarray analysis method detailed below.
Clone sequencing strategy has been used to verify the selected methylation markers by microarray analysis method in a whole-genome wide (Sangon Biotech Incorporation, Shanghai, China).
For human Affymetrix arrays, we read CEL files and then corrected background, summarized and normalized the data by robust microarray analysis method.
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The drastically different structure of these high-sensitivity transcriptome profiles requires adaptation or even redevelopment of the standard microarray analysis methods.
We thank Lorenz Wernisch for helpful discussions on microarray analysis methods.
A flowchart of microarray analysis methods is outlined in Fig. S1.
Microarray analysis methods produce large sets of gene expression data that can be exploited for novel insights into the fundamentals of molecular biology research.
RIP-chip violates important assumptions of many microarray analysis methods.
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