Sentence examples for microarray validation method from inspiring English sources
Quantitative PCR was selected because of its universal use as a microarray validation method [ 10, 11, 18- 21], enhanced dynamic range [ 18- 20], and ease of use with limited sample sizes for evaluating expression changes for several hundred genes.
In this case, poor selection of the target validation method (microarrays after miRNA modulation) based on the assumption that most targets would have destabilized mRNA masked the functionality of these far more common sites (up to 50% of all miRNA-miRNA interactions).
In the MAQC project, the quantitative accuracy of several non-microarray devices was checked, then quantitative RT-PCR (Taqman system) was selected as a validation method of microarray data.
Real-time quantitative PCR (qPCR) is considered a 'gold standard' for quantification of gene expression and has been widely employed as a validation method for microarray studies.
Because CIPers can also include a barcode, they could be used for microarray validation, or amplicon tracing with sequencing methods [17], [44] allowing for longer read lengths [38].
All DMRs positively validated in the microarray validation experiments were subjected to initial clinical validation screening.
Interestingly, microarray validation by RT-qPCR confirmed the contrasting expression of miR-31.
Microarray validation ΔCt's were calculated as 2−(Ct GOI –Ct HK ) (GOI, gene of interest; HK, housekeeping gene).
Fig. 3 Framework validation method.
Input prediction model, validation method.
