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Exact(4)
The embryos were washed several times in 75 μl micro drops of M16 medium and cultured 24 h to 8-cell in 50 μl micro drops of M16.
The zona free embryos were washed by passage through several 75 μl micro drops of M16 and placed in M16 holding drops.
The zona pellucidas were removed from the FVB and C57 8-cell embryos by placing them into 100 μl micro drops of a 0.5% protease (Sigma Chemical Company; St . Louis MO) in M2 solution for 10 min at 37°C.
Individual C57 blastomeres were generated from C57 embryos by placing them into 100 μl micro drops of Mg++ and Ca++ free Dulbecco's PBS (Gibco; Carlsbad, CA) plus 0.6% BSA (Sigma Chemical Company; St . Louis MO) for 15 to 20 min at room temperature.
Similar(56)
After centrifugation of the formed cloudy mixture, a micro-drop of the aqueous phase was sedimented at the conical bottom of the centrifuge tube.
A non-charged complex of cadmium with ammonium diethyldithiophosphate (DDPA) was produced and extracted on-line into a 60 μL micro-drop of di-isobutyl ketone (DIBK).
In conventional DLLME, the sedimented phase is a micro-drop of a chlorinated organic solvent that is not compatible with RP-HPLC.
Flushed embryos were maintained at 37°C in 5% CO2 in micro-drops of M16 medium under oil for 2 days to allow hatching from the zona.
ESCs were affixed to the bottom of micro-drop cultures and treated with differentiation factors.
The high cooling rate of metal surface vitrification method was achieved by using the combination of micro-drops and improved heat exchange via direct contact with a metal surface.
The analysis concerning the micro-drop influence on the sensor operation has been completed by means of coupled thermo-mechanical FEM simulations.
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