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For magnetization measurements, 3 micro litres of the fluid was dropped over to a quartz substrate and the base liquid was allowed to evaporate.
The system uses a ready to use test strip into which a sample volume of 200 micro litres of serum or lithium heparin plasma is pipetted.
Ten micro litres of each lemon grass essential oil treated, lemon grass essential oil vapour treated and untreated Candida cells suspension was mounted on a glass substrate.
Five micro litres of this sample was withdrawn using a micro pipette and a Leja "chamber" slide (20 μm deep and 5 μl volume) (Leja Products B.V., Nieuw Vennep, The Netherlands) was filled.
Five micro litres of incubated TA98 or TA100 cells (16108 cells in 0.1 ml), OS-EL (17.5 ml), and the mutagen reagent (2.5 ml) were mixed in a 50 mL sterile test tube with a cap.
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One micro litre of DNA was added into the reactions for the detection system.
PMN were counted in 20 micro litre of milk in light microscope after Newman staining according to a modified version of the IDF standard (IDF 148-1/ISO/DIS 148-1/ISO/DIS
Hundred micro litre of virus/phage suspension was fixed with 10ul fixative (10% formaldehyde and 5% glutaraldehyde in deionized water) to give a final concentration in the virus/phages suspension of 1% formaldehyde and 0.5% glutaraldehyde.
One micro litre of colony materials from the opaque zone of the colonies was streaked on XLD + N and BG, and incubated at 37 ± 1°C for 24 ± 3 h.
One micro litre of serum diluted in PBS/0.3% bovine serum albumin (BSA) (PA buffer) was incubated over night with 1 mg Sepharose-immobilized protein A (GE Healthcare, Chalfont, St. Giles, UK) in a final volume of 800 μl.
Nine and a half micro-litres of master mix was added to each well.
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CEO of Professional Science Editing for Scientists @ prosciediting.com