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Based on the previous findings of impaired vessel integrity in Cd34−/− mice [11], we also assayed vascular integrity in tumor tissues by injecting mice with fluorescent carbocyanine dye prior to sacrifice.

Transgenic mice with fluorescent reporter genes also represent an interesting alternative (92, 93).

We inoculated mice with fluorescent beads either by intravenous or airway routes and compared the proportion of phagocytic cells in different lung subsets 4 hr after bead inoculation.

Jamon proposed that these trails develop from following scent left during earlier visits; he dusted wood mice with fluorescent pigments, which revealed trail-following to be non-random.

Enteroendocrine cells have historically been difficult to study as they are found scattered within the intestinal epithelium, but recent advances in labelling specific cell populations in transgenic mice with fluorescent reporters have enabled live cell identification and purification.

To assess the ability of primary splenic DCs and MZ macrophages to retain lipid antigens arriving to the spleen we injected mice with fluorescent particulate lipids and 2 h later we analysed antigen retention by splenic cell populations by flow cytometry.

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We have developed a novel method to quantify beta-cell proliferation and islet formation in the intact pancreas (without sectioning) using transgenic mice with fluorescent-tagged beta-cells combined with an automated computational analysis using a macro written for ImageJ, free software available at the NIH website.

Bright fluorescent dots were observed in lung sections from mice treated with fluorescent MIR2911.

We compared the tissue biodistributions of subcutaneously or intravenously injected Smaducin-6 in mice administered with fluorescent dye TAMRA (carboxytetramethylrhodamine -conjugated Smaducarboxytetramethylrhodamine -conjugated

During myogenesis and postnatal development, the shape of mitochondria is also remodelled: in an elegant mouse model with fluorescent mitochondria it was shown that in young mice mitochondria of the extensor digitorum longus (EDL) muscle are shaped as elongated tubules oriented along the long axis of the muscle fibre, whereas in adult mice mitochondria are punctuated and organised into doublets.

These findings come from in vivo two-photon studies of transgenic mice with green fluorescent protein expressing microglia, which show that microglial cell bodies remain stationary while their branches are highly dynamic.

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