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Platelet-rich plasma obtained from healthy (PRP) or tumor-bearing (TPRP) mice was applied to dorsal, full-thickness wounds on diabetic mice.

Male and female 17-week-old C57BL/6 mice were housed in groups of six, and a single episode (40 cycles) of non-invasive axial loading, engendering 2,200με on the medial surface of the proximal tibiae in sample mice, was applied to right tibiae on alternate days for two weeks.

In order to accurately follow the natural diurnal rhythm of glucocorticoid secretion in HR, IR and LR mice without interfering with the activation of the HPA axis by repeated handling and blood sampling, a non-invasive technique to monitor adrenocortical activity by measuring corticosterone metabolites (CM) in the faeces of mice was applied [31], [32].

PMA (1 μg in 200 μl acetone), or acetone (200 μl) for control mice, was applied to the dorsal surfaces of shaved mice.

A technique similar to that used for conscious mice was applied by placing the head and trunk in an in-house-made container, composed of a 50 ml plastic centrifuge tube with holes for ventilation, leaving the hind legs free.

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Paw pressure test in rats, hot plate and writhing test in mice were applied to appraise the analgesic effects.

Human MCF-7 breast cancer in nude mice and animal model of P388 lymph node metastases in Kunming mice were applied to investigate the therapeutic effects.

Both groups of conditioned medium (from either WT or KO mice) were applied to the Transwell assays and cell scratch assays to compare their effects on cell migration of BMSCs.

Secondary antibody (100 μl Dako horse radish peroxidase conjugated rabbit anti-mouse) was applied for 30 min at room temperature.

Afterwards, an AlexaFluor594-labelled secondary antibody against mouse was applied at a concentration of 1 : 200 and incubated for 1 hour (Alexa Fluor 594 goat-anti-mouse IgG1; Invitrogen Corp., Karlsruhe, Germany).

Since the time points of blood sampling and plasma glucose measurements varied slightly from mouse to mouse, interpolation was applied for the inter-group analysis at predefined time points.

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