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A grey-scale photographic image of the mice was fused with the bioluminescent images.

Cells from spleens of immunized mice were fused with SP2/0 meyoloma cells as described previously [68].

Three days after the last injection, spleen cells from the immunized mice were fused with myeloma Sp2/0 cells [12].

Three days after the final immunization, cells from the lymph nodes of the immunized mice were fused with P3-U1 myelomayeloma cells in the presence of 50% (w/v) polyethylene glycol (PEG4000) (Wako).

Splenocytes from the immunised mice were fused into SP2/O cells.

Spleen cells from immunized mice were fused with Sp2/0 mouse myeloma cells at a ratio of 5 1 in the presence of 50%% (w/v) PEG 4000.

Spleen cells from immunized mice were fused with the non-secretory myeloma P3-X63-Ag.8.653 by additiof of polyethylene glycol (PEG) and were subjected to HAT selection.

To generate monoclonal Abs against huLTF, splenocytes from huLTF-immunized mice were fused with SP2/0 murine myeloma cells and cells were subsequently cultured in 96-well plates.

Following MAR3-MBP protein boosts, 50% endpoint titers to target were in excess of 1/10,000, and spleens of two mice were fused.

Three days after the last injection, spleen cells from the mice were fused to SP2/O-AG14 myeloma cells (ATCC) to generate hybridomas.

Three days after the final injection, spleen cells of the immunized mice were fused with a myeloma cell line using 50% (w/w) polyethylene glycol (Roche Diagnostics, Basel, Switzerland).