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Quantification of the parasite load in murine tissues was done with 2 separate quantitative PCRs (QPCRs): (i) one PCR targeting kDNA to quantify Leishmania as described by Nicolas et al [22] and (ii) one PCR targeting the single copy gene neurotrophin 3 of mice to quantify the amount of murine cells present in each sample (Table 2).
Angiogenesis was examined by intraperitoneal injection of 5-bromodeoxyuridine (BrdU) in mice to quantify new brain vessel growth.
We previously used doxycycline (Dox -inducible TetO-H2B-GFP reporter mice to quantify the proliferative history of SCs Dox -inducibleChakkalakal et al., 2012).
We obtained longitudinal images by micro-CT of CIA mice and compared them with images from untreated control mice to quantify the changes in bone volume and density induced by disease.
To further investigate HDGF expression in melanocytes, primary melanocytes were isolated from neonatal skin of HDGFTyr, wildtype and HDGF-/ mice to quantify the elevated HDGF-expression in HDGFTyr melanocytes by immunofluorescent stainings.
To validate the significant changes induced by 5-AZA-CdR in GEP of cancer cells, real-time RT PCR assays were performed in neoplastic tissues, from control and 5-AZA-CdR-treated mice, to quantify the expression of 11 genes, randomly selected for their differential up-/downregulation by 5-AZA-CdR.
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To overcome some of these issues, Schwanhausser et al. used mouse fibroblasts to quantify and analyse global mRNA and protein levels along with their associated half-lives, transcription, and translation rates.
Cryo fixation is thought to give a truer representation of biological structures, and here we use rapid, high-pressure freezing on adult mouse neocortex to quantify the extent to which these two fixation methods differ in terms of their preservation of the different cellular compartments, and the arrangement of membranes at the synapse and around blood vessels.
We decided, therefore, to produce primary cultures of MGCs from knockout mice in order to quantify MHC class I expression by flow cytometry.
Non-invasive optical imaging of living mice, allowed us to quantify shERα activity in vivo.
Six random view fields (under 40x magnification) of H&E or CD31 staining slides from 6 individual mice were imaged to quantify the blood vessels or human derived lumens formed in the cell sheet constructs in vivo, respectively.
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