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In another experimental set, we added BGT-biotin to whole muscle lysates of untreated mice to label the total population of AChRs [37].
Tbr2 Cre mice were bred with Ai9-tdTomato reporter mice to label all neurons derived from the Tbr2+ IPCs.
Thus, we first crossed in the previously described conditional Rosa reporter transgenic line (R26R-YFP) (Lagace et al. 2007; Soriano 1999) to obtain fRIM1/POMC-cre+/R26R-YFP mice to label cre recombinase activity in the presence of fRIM1.
To further determine the location of A11 immunostained oligomers, we performed co-staining of brain sections from 24-month-old APP23/wt mice to label nuclei and senile plaques.
Retrograde tracer injections were made in either the contralateral M1 or ipsilateral M2, S1, and S2 of 6- to 7-week-old mice, to label corticocortical projection neurons in M1; brain slices were prepared 3 7 days later and imaged as described below.
Similar(55)
Serum taken immediately from biotin-injected mice failed to label cells of untreated mice, demonstrating the rapid clearance of biotin from circulation (Fig. 1A).
Radial glia specific BLBP promoter-DsRed2 DNA was electroporated into Dlx5/6-CIE mice cortices to label radial glia with DsRed2.
Thus, the bitransgenic mice appear to label cardiomyocytes (and their progeny) specifically, allowing us to conclude that c-kit+/GFP+ cells in that model arose from dedifferentiated cardiomyocytes.
Following a thorough buffer wash, cells were incubated with a 1 160 000 dilution of mouse monoclonal antibody (G9) followed by a 1 100 dilution of rhodamine red anti-mouse IgG to label γ-tubulin, and a 1 100 dilution of rat monoclonal anti-α-tubulin followed by a 1 100 dilution of fluorescein anti-rat IgG to label microtubules.
Cells were then washed and incubated on ice with FITC-conjugated goat anti-mouse immunoglobulin to label the cell-bound mAbs or liposomes.
We used a Cre-dependent GFP expression strategy in mice to genetically label the mRGCs.
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