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APMV serotypes 4 and 9 also were detected in the brain in two mice each at 3 dpi.
For the experiments cells of two independent isolations (6 mice each) at passage two to five were used.
We used three mice each in PMCAO groups that exhibited neurological grades G1 and G2 (Ohtaki et al., 2006) and three mice each at random in sham groups for the subsequent downstream analysis.
Thus, consistent miRNA profiles from 3 mice at 7 dpi and 4 mice at 15 dpi, together with consistent mRNA profiles from 4 mice each at 7 and 15 dpi were analyzed (Additional file 1: Figure S1).> -wrap-foot> Note: Histopathologic scoring to determine the homogeneity of lung damage at 7 and 15 dpi.
The tumor model animals were randomly divided into five groups of 10 mice each at 24 h after tumor inoculation, group 1 (Control, 1% Tween 80 solution, 0.2 ml/20 g/day), group 2 (the positive control, CTX, 20 mg/kg/day), group 3 (the low dose of the TAFU, 20 mg/kg/day), group 4 (the medium dose of the TAFU, 40 mg/kg/day), group 5 (the high dose of the TAFU, 80 mg/kg/day).
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Visual-guided behavior was tested at 100, 1, 0.1, 0.01, and 0.001 cd/m and timed performances from 20 trials (four sessions of five trials each) for each mouse at each light-intensity were averaged.
Finally, the PCDMs were normalized to a standard section and averaged for each group of five mice at each of the five levels (Fig. 3).
Vacuoles were scored in testes from at least three mice at each age.
The genotype of each mouse at each of the microsatellite markers was determined.
For each mouse at each time point, the concentration in brain (ng g−1) was divided by the concentration in blood (ng mL−1) to give a brain/blood ratio.
The numbers of in situ and invasive cancers identified in each mouse at each age are shown in Figure 3 (a to g correspond with mouse A to mouse G) in a stacked column plot.
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