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We systematically examined the placentas of Pkd1+/+, Pkd1+/− and Pkd1−/− mice at gestational ages E10.5 through E15.5 in both mixed (129Sv/BL/6) and congenic C57BL/6 backgrounds.
Gamma irradiation inactivated mouse embryonic fibroblast (MEF) feeder cells isolated from the embryos of ICR mice at gestational day 13.5 were purchased from Invitrogen (Carlsbad, CA).
Gamma irradiation inactivated mouse embryonic fibroblast (MEF) feeder cells isolated from the embryos of ICR mice at gestational day 13.5 were purchased from Invitogen (Invitrogen, Carlsbad, CA).
Neural stem cells were obtained from mice at gestational day E14.
In detail, pregnant mice at gestational day 14 or 17 were treated subcutaneously with RU486 (mifepristone) 2.5 ml/kg/5 ml at 1 00 PM.
We observed an enrichment of DE methionine cycle genes in fetal livers of male C57BL/6 J mice at gestational day 18, of which the mothers had been exposed to 50%% food restriction (expression data obtained from GSE49964).
Similar(54)
Methods: Embryonic pancreas was harvested from mice at serial gestational ages and immunohistochemical analysis was performed for retinoic acid receptors (RAR-α, RAR-β, RAR-γ), and retinoid X receptors (RXR-α, RXR-β, and RXR-γ).
Pregnant mice at the gestational ages of 12 14 days and non-pregnant mice were intranasally inoculated with 2×106 plaque forming units (PFU) of wild type virus and 150 PFU of mutant virus (Table 1), respectively, after anesthesia with ketamine (100 mg/kg) and xylazine (10 mg/kg) by intraperitoneal injection.
The mixed gestational mouse cultures have two times as many cholinergic interneurons as single E14.5 cultures.> -wrap-foot> Percenumberber ± standard deviation of immunohistochemically identified interneurons observed in CPu neuronal cultures obtained from embryonic mice at different gestational ages.
Similar observations were made for the parvalbumin interneuron.> -wrap-foot> Percenumberber ± standard deviation of immunohistochemically identified interneurons observed in neuronal cultures obtained from embryonic mice at two gestational ages E12.5 or E14.5.
Primary neurons were isolated from mouse embryos at gestational day 17 18 and cultured as described previously.
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