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To determine expression levels of the genes, iWAT was acutely dissected from the mice at indicated time points after treatment.
Vaginal secretions were collected from infected mice at indicated times.
EGCG was administered intraperitoneally into mice at indicated doses and time points, and mice were monitored for survival for up to two weeks.
Primary keratinocytes were isolated from mice at indicated ages as described in [36] and either directly applied in a functional assay or seeded on a mixture of 30 µg/ml Vitrogen (bovine collagen type I; Cohesion) and 10 µg/ml fibronectin (Invitrogen -coated plastInvitrogen -coated growth medium containing 8% chelated FCS (Gibco) and 45 µM Ca2+.
105 CD8+ Teff were labeled with 1.25 µM CFSE and cultured with CD4+foxp3+ cells (FACS-sorted from foxp3GFP mice) or CD4+CD25+ cells (MACS-selected from C57BL/6 mice) at indicated ratios, along with 105 T-cell-depleted, irradiated (444 cGy) splenocytes and 1 µg/ml anti-CD3 mAb.
The engrafted skin grafts were taken down from tolerant mice at indicated time points.
Similar(49)
For immunofluorescence analyses, peritoneal cells were harvested from PBS or LPS conditioned-mice at indicated time point post-CLP, and spun on glass slides through cytospin.
ALT blood level of control (WT) and YapCKOAlb-Cre (KO) mice measured at indicated days after CCl4 treatment.
The body weights of the mice were measured at indicated time point.
(A) snPt1 or snPt8 was injected intravenously into mice at the indicated doses.
Cells were centrifuged, washed, resuspended in sterile PBS and administered to mice at the indicated doses.
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