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Blood glucose levels were not significantly different between TGwt and TGmut mice at baseline (Table 1), both groups showing a similar decrease of 60%to70%0% in blood glucose values following insulin pretreatment prior to FDG injection (Table 1).

Myocardial and skeletal muscle glucose uptake was assessed with 2-[18F]fluoro-2-deoxyglucose 2-[18F]fluoro-2-deoxyglucose 2-[18F]fluoro-2-deoxyglucose 2-[18F]fluoro-2-deoxyglucoseG2 mutant (TGmut) mice at baseline and 1 week later, 30 min following acute insulin.

We observed very few nitrotyrosine positive cells in colons of TLR2−/− and WT mice at baseline (Figure 7C).

In adult ATP8B1-deficient mice at baseline, those of B6 background had lower serum cholesterol levels, higher serum alkaline phosphatase levels, and larger livers.

However, in contrast to Backhed, we did not observe increased body weight, % fat or fat mass in age-matched CONV mice versus GF mice at baseline.

In contrast, the expression of lipocalin (Lcn2) was enhanced in PM isolated from CLP mice at baseline, as compared to sham PM, and the expression of this gene was further augmented in CLP PM stimulated with LPS (panel D).

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23 The enzyme necessary for PD1 synthesis, 15-LOX was not induced in PHIL-mice and, thus, PD1 was barely detectable in PHIL-mice at baseline or during colitis suggesting an important role for these leucocytes in PD1 synthesis in this myeloid predominant acute experimental colitis model.

Conscious low-resolution nuclear magnetic resonance imaging was used to measure body composition of each mouse at baseline, 6, 12, and 22 weeks using an EchoMRI 3-in-1 Body Composition Analyzer for Live Small Animals (Mice)(EchoMRI, LLC, Houston, TX) [ 16].

Conscious low-resolution nuclear magnetic resonance imaging was used to measure body composition of each mouse at baseline, 6, 12, and 22 weeks using an EchoMRI 3-in-1 Body Composition Analyzer for Live Small Animals (Mice) (EchoMRI, LLC, Houston, TX, USA) [ 27].

Included were (1) scans from mice studied at baseline prior to any treatment and (2) any subsequent scans from mice enrolled in control groups not receiving any drug substance.

All three groups of mice injected with vector and followed for an additional 6 weeks had more atherosclerosis than mice analyzed at baseline (Fig. 3b).

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