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In Cx57-deficient mice, application of cobalt led to the same shift in spatial tuning (Fig. 6B, p<0.0019, KS test) in a way that was not significantly different from its effect in wild-type mice (p>0.66, KS test).

In wild-type mice, application of 200 μM 4-C mC induced measurable changes in Fura-2 signal.

Therefore, due to the short life span of p53 knockout mice, application of this model is very limited.

The authors demonstrated that, in brainstem slices from Mecp2 mutant mice, application of LM22A-4 significantly reduced neuronal hyperexcitability in respiratory reflex pathways.

In these mice, application of diphtheria toxin resulted in an efficient depletion of monocytes from the circulation and of macrophages from the ovary and the kidney.

In mice, application of antigen to slightly barrier-disrupted skin, and even intact skin, can be sufficient to induce therapeutic effects.

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Since weights of the Atm-/-mice were less in comparison to wild-type mice, initial application of 50 μl hydrochloric acid may have shown a high mortality rate in Atm-/-mice due to a higher volume per body weight.

In comparison with control mice, the application of IFN-γ significantly prolonged survival time and reduced the penetration of Salmonellae into intestinal epithelial cells, development of bacteremia and mortality rate (Degre and Bukholm, 1995).

In ear skin of p53 wild-type mice, topical application of thymidine dinucleotide induced a 4 5-fold increase in epidermal melanin content after 3 wk, but in p53 knockout mice thymidine dinucleotide application caused no detectable increase in melanin.

Despite the conclusive demonstration by Russell and colleagues in the 1970s that the chemical N-ethyl-N-nitrosourea (ENU) is a highly effective mutagen in mice, the application of phenotypic-driven mutagenesis as a method to study mammalian biology proceeded slowly.

In the same neurons from WT mice, focal application of GBZ failed to reveal a tonic current (absolute 4.8±1.2 pA, normalized 0.2±0.1 pA/pF) (Fig. 3C E), as described previously for layer 2/3 pyramidal neurons in these WT mice [21].

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