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Exact(9)
In brief, sample (1.5 mg) was solubilized in anhydrous 1.25 M HCl/MeOH (1 ml) and then heated at 80°C overnight.
Briefly, the polyester (40 to 360 mg) was solubilized in water-saturated ethyl acetate (10 ml); then, the neutral oil (0.1 to 0.9 ml) containing Doc (2 to 18 mg) was further added to the organic mixture.
Magnetic nanostructure Fe3O4@C16 (200 mg) was solubilized in 1 mL of chloroform and oriented in magnetic field, and 100 μL analytical standard of eugenol (E) (Sigma-Aldrich) and respectively, limonene (L) (Sigma-Aldrich) were added and mixed until complete evaporation of chloroform was reached.
Type II collagen (4 mg) was solubilized in 1 ml of 0.05 M acetic acid.
Compound 1 (0.259 mmol, 76 mg) was solubilized in anhydrous ethanol (6 mL) and transferred to a sealed tube.
The protein (2 mg) was solubilized in rehydration buffer containing 6 M urea, 2% CHAPS, 20 mM DTT, 0.5% IPG buffer, and a trace amount of bromophenol blue.
Similar(51)
Just prior coupled, Ig-438 (30 mg) were solubilized in 15 ml of NaHCO3 (0.1 M, pH 8.3) containing 0.5 M NaCl.
Samples of dialysed GG and GG 30, about 4 mg, were solubilized in 0.7 μL of D2O.
EtOH and CHCl3 extracts (10 mg) were solubilized in 500 μL of DMSO and from these stock solutions, several dilutions were prepared to achieve concentrations between 200 and 3.13 μg/ml.
Brain plugs (25 mg) were solubilized in 500 μl of chilled measuring buffer [10 mM potassium Hepes (pH 7.4), 40 mM KCl, 1% Tween 20, 2 μM oligomycin, 1 mM PMSF, 10 mM potassium fluoride, 1 mM sodium vanadate and 2 mM EGTA] using a Teflon microtube pestle applying five strokes followed by sonification (micro tip, 3×10 s pulses).
Briefly, 1 mg mL−1 Aβ42 was solubilized in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) (Fluka, Sigma Aldrich Company Ltd., Dorset, UK), vortexed for 60 s and bath sonicated for 60 s.
Related(20)
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