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Takahashi et al.[ 32] reported that a considerable number of genes (8 out of 24 genes) are frequently methylated in GBC in comparison with chronic cholecystitis, varying in methylation frequency between 4% and 80%, with p16 having the lowest rate [ 32].

There was no significant difference in the methylation frequency between the histologically normal and BPH groups.

χ2 test was used to compare the methylation frequency between tumors and tumor adajcent normal tissues.

Among these five genes, there were no significant differences in methylation frequency between premenopausal and postmenopausal subgroups and T1 and T2/T3 subgroups.

Differences in methylation frequency between BL and DLBCL, especially in Leprel2 and P4HA2, led us to address the possible utility of these genes as biomarkers to differentiate between the lymphoma types.

For example, Fackler and colleagues [ 37] reported TWIST1 methylation, as detected by MSP, to occur more frequently in IDC (15/27, 56%) compared with grade 3 DCIS (7/18, 39%), grade 2 DCIS (3/12, 25%), and grade 1 DCIS (2/14, 14%), and the difference in methylation frequency between IDC and combined grade 1 and 2 DCIS was statistically significant (P = 0.01).

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Individual cell lines varied in methylation frequency from 5/17 (Hep3B) to 15/17 (RAJI) and colon cancer and leukemia appeared to have the most methylation.

The methylation frequency of APAF-1 (DAPK-1) was 100% (77%) in TCC and 100%3333%) in RCC.

The methylation rate of different tumor suppressor genes in cancers of the digestive tract (stomach, colon, pancreas and gallbladder) has been studied, and show a high methylation frequency of CDH1 (77.8%) [ 46].

Superficially, the corresponding picture for the CDX1 gene was not as clear-cut, with a true basal CpG methylation frequency of 8.2 2.5 = 5.7%, going up to 11.1 3.0 = 8.1% in 6TG-treated T84 cells.

The prediction of the MGMT methylation status using MGMT-STP27 in a cohort of grade II and III gliomas (T-Glioma-II/III) with HM-450K data, determined a methylation frequency of 79%% (32/42) in grade III glioma and 86 % (25/29) in grade II.

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