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There were no significant differences in the methylation rate between the cancer cell lines and primary tumours in either the m2a or the m2b region.
In the analysis of p16 and p14, it was noted that they presented a high methylation rate (between 40% and 60%) for carcinoma of the biliary duct, GBC and for primary sclerosing cholangitis.
Similar(58)
The methylation rates between tumor-bearing nude mice groups were analyzed using the Fisher's exact test.
In contrast, methylation rates between birth and postnatal day 4 were stable in term infants (figure 1).
The region is defined as a DMR if p < 0.05 (chi-square test) and the difference in methylation rates between cancer tissue and normal tissue is >0.1.
Briefly, they identified methylated DNA in eight breast cancer cell lines and one normal breast cell line, and in addition they compared methylation rates between parental MCF-7 cells and MCF-7 cells that had undergone epithelial to mesenchymal transition.
Then, the seed is prolonged by checking CpG dinucleotides one-by-one according to the results of the t-test and chi-square test of the difference in methylation rates between samples.
The methylation rate increased significantly between postnatal days 0 and 4 in preterm infants but remained stable in term infants.
Differences in promoter methylation rate were analysed between cancer and non-tumor tissues using Fisher's exact test.
Then, we analysed the relationship between the methylation rate at these CpG sites on postnatal day 4 and subsequent complications.
Logistic regression analysis was performed to investigate the relationships between the methylation rate at 4 days as a dependent variable with later complications in the neonatal period as independent variables.
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