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Using previously described methods, we confirmed that the cell lines contained a portion of the MCMV IE promoter-lacZ (407 bp) [21].
Using different, complementary biochemical and biophysical methods, we confirmed the predicted disordered nature of Henipavirus NTAIL and PNT and show that they belong to the PMG subfamily within the class of IDPs.
Using standard stereological methods we confirmed that the core-volume of virions attached to the cell surface or of incoming/cytoplasmic cores was about 2-fold larger than that of isolated, intact, particles (Table 2).
Using both biophysical and biochemical methods, we confirmed that the synthetic and wild type gene products expressed in native E. coli competent cells were identical and by functional assays validated the neurotrophic and antiangiogenic efficacies of the proteins.
Using independently generated anti-neurexin antibodies (see methods), we confirmed the presence of neurexin in larval NMJs, and extended this analysis to embryonic NMJs (22 24 h AEL; Fig. 1).
In parallel, using the same methods we confirmed that microRNAs are also detectable in other body fluids, such as urine, saliva, amniotic fluid and pleural fluid (Figure 2B and data not shown).
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Using our sigmoid fitting method, we confirmed previous two major findings regarding the relationship between balloon filling volume and geometric volume and surrounding pressure [14, 15].
As it is a new method, we confirmed the evaluation endoscopically through a small trephined hole in the lateral sinus wall.
With this clustering method, we confirmed the inhomogeneity in this system, defined cell clusters, quantified and localized them, and determined the cell density within clusters.
Applying small-angle X-ray method, we confirmed that at transformation from IMC to IPEC Ag0 structure of nanocomposite, the relative heterogeneity level is augmented, while effective size of the region heterogeneity goes down.
In this way, we clarified the differences between conventional methods and our presented method; we confirmed the advantage of the presented method in identifying the optimal global allocation of defensive resources.
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