To use the comparative CT method, we ascertained that the efficiencies of the target and endogenous control amplifications were approximately equal.
To demonstrate an insensitivity to template input amounts for this particular method, we ascertained the reliability of our test over a wide range of mtDNA copies, from 10 to 10. Results are displayed in Supp. Figure S1.
Accordingly, by studying the properties of these methods alongside those of the other contending methods, we have ascertained whether and when their theoretical inconsistency may lead to inferior performance.
We propose an optimized matching method where we ascertain family configurations that are most informative, while making sure that relevant features, such as the risk to relatives of cases, are similar in the sampled data and the full cohort.
Using the methods we applied above to ascertain fixed AluY regions, we identified 552 polymorphic AluY regions based on the AluY loci in dbRIP [ 24].
In order to develop meaningful and beneficial educational methods, we first sought to ascertain the education needs and preferences of the local practicing pediatricians and pediatric residents in our community with the assumption that these findings can help inform and shape our future efforts and also inform others interested in addressing this educational need.
We ascertained dialysis using the method described above, but selected codes explicitly referring to dialysis treatment (see Additional file 1).
However, we ascertain that 1D methods should be used with caution for the water-cooling FW design.
Points to consider, regarded relevant for individual treatment decisions as well as their assessment methods, were ascertained by experts' consensus applying the Delphi technique.
Although we used robust methods to ascertain disease outcomes, 22 preferential diagnoses in people known to have chronic kidney disease may have resulted in overestimation of hazard ratios.
