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Thus, alternative methods of proliferation assessment are of interest, in particular if they could be used in routine practice.
As discrepancies are common between these two methods of proliferation evaluation, [ 42] a parallel cannot be drawn between this study and our results on this variable.
For these reasons the development of non-invasive, reproducible and validated methods of proliferation measurement will be a major advance for the evaluation of anti-neoplastic agents and for identifying non-responders early in their treatment so that they can be offered alternative and possibly more efficacious therapies.
Therefore, the aim of this study was to examine whether hypoxia can influence stem-like properties of ovarian cancer cell lines in vitro with the methods of proliferation assay, cell cycle analysis, infiltration assay, colony formation assay, sphere formation assay, SP, FACS and Western blotting.
Consequently, this study was conducted on a large, well-characterized series of early (stage I through III) invasive BC with long-term follow-up to study the prognostic value of MS and Ki-67LI, as methods of proliferation assays, in different BC molecular classes.
Similar(55)
Thus, Yang's data support Ki-67 staining of core biopsies as an adequately reliable method of proliferation assessment for prognosis.
Simple linear regression was performed to obtain correlation coefficients for the two methods of measuring proliferation.
The measurement of the fraction of cells engaged in chromosomal DNA synthesis (the S-phase fraction (SPF)) has become one of the standard methods of assessing proliferation.
As previously discussed, each of the major methods of measuring proliferation has revealed evidence of correlations of proliferative rate with recurrence and overall survival [ 1, 2, 11, 15, 27- 29].
These data were validated with a single dose of CX3CL1 and a positive control (PDGF-BB) using two alternative methods of measuring proliferation: Ki67 staining and total cell counting.
A recent study compared methods of assessing proliferation: 'hotspot' Ki-67 assessment in one field; field average over 10 consecutive fields using digital imaging analysis; and MC (Goodell et al, 2012).
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