Newer methods of labeling involving bifunctional chelating agents (which encompass the "3+1" ligand system, Tc(CO 3+1-containing CO 3+1-containingnonichelatesde, water-soluble phydrazinonicotinamideTc-carrying moieties) have added a new dimension for the preparation of novel technetium compounds.
Results and Discussion - Insertion of the fluorophores into LUVs: It is stated that no detectable difference is found comparing two methods of labeling referring to Figure 4.
2) what is the minimum amount of total RNA required to perform a representative amplification? 3) are the direct and indirect methods of labeling the hybridization probe equivalent?
In the lecture portion of my course, the students learn about several different methods of labeling nucleic acids, including nick translation and 3′ end labeling via the "fill-in" technique using the Klenow fragment of E. coli DNA polymerase I.
Moreover, as most current methods of labeling polyA RNA for gene expression analysis show a marked drop off in efficiency with distance from the polyA tail, array features corresponding to 3' UTRs are more likely to show above background signals after hybridization with labeled RNA.
The aim of this study was to explore novel methods of labeling proteins for in vivo localization studies that do not perturb protein function or structure.
