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"Modern methods of cloning genes will still be patentable".
Human reproduction with the methods of cloning is expressly prohibited in Section 1455 [ 27].
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Advanced Cell Technology, a company that wants to clone human embryos to obtain stem cells for disease treatments, licensed a patent from the University of Massachusetts on its method of cloning.
Alphaclone gives equal weight to the top 10 holdings, which they believe is a better method of cloning.
Information regarding antigen specificity, species of origin, method of cloning, and other relevant serological properties was correlated with the sequence data.
That study also utilized the Gateway method of cloning and the commercially available ORFeome library.
The method of cloning the double-stranded shRNA sequence is described in the manufacturer's protocol.
In our hands, using the Gateway method of cloning and the commercially available nematode ORFeome library we obtained a 59% success rate.
Therefore, we adopted an old nested deletion method of cloning [ 30] to improve the sequencing yield.
A rapid and reliable method of cloning target DNA is keenly desired.
A rapid and reliable method of cloning native DNA sequences into desired plasmids are highly desired.
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