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We focus here on selected examples that illustrate this breadth, and review the application of methods in single particle cryo-EM and cryo-electron tomography to progress in the structural biology of CRISPR systems, visualization of small molecule drugs in membrane proteins, in situ visualization of bacterial nanomachines, and the analysis of antigen antibody interactions to drive vaccine design.
The accuracy of the CASA methods in single channel speech separation depends on the correctness of the pitch frequency estimation of two simultaneous speakers because the proper mask in the T-F domain for the speech separation is produced in association with the estimated pitch frequency.
Thus, red-shifted FRET pairs are preferable for detecting protein-protein interactions by donor-based FRET methods in single living cells.
In conclusion, we have shown that red-shifted FRET pairs are preferable for detecting protein-protein interactions by donor-based FRET methods in single living cells.
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Her lab has pioneered foundational experimental and computational methods in single-cell genomics, working toward greater understanding of the function of cells and tissues in health and disease, including autoimmune disease, inflammation, and cancer.
This study experimentally determines the tensile failure load of the nanocomposite adhesive – obtained by adding nanostructures to the adhesive – using four different methods in single-lap joints.
Using a suite of advanced techniques in molecular and bacterial genetics, coupled with cutting-edge methods in single-cell imaging, the Rego lab will track the metabolic activity in individual bacterial cells before, during, and after exposure to drugs used to treat TB and assess how the metabolic state of an individual bacterial cell correlates with its growth rate and infectivity.
Digenic interactions/epistatic QTLs (LOD >5.0) using ICIM-EPI method in single environments E1, E2, and E3.
The satisfactory quantitative results demonstrated the feasibility of the method in single and mix food antiseptics analysis.
The conventional method in single particle analysis of heterogeneous sample with multiple conformations is to perform 2D or 3D classifications to try to separate different conformations into independent classes.
In gastric cancer, a few Japanese surgeons reported efficacy of this method in single arm studies [ 5, 6].
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