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Lactate uptake is the major mechanism of renal lactate removal and appears to be essentially confined to the cortex [ 5, 14], as shown by radioisotopic methods in isolated, perfused rat kidney [ 14].
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One of the isolated variants cleaved the Gln-containing peptide substrate more efficiently in vitro, proving the efficiency of our method in isolating engineered proteases with desired substrate selectivity.
Besides indicating that the enriched library is depleted in housekeeping genes, the GO annotation also shows that this library is enriched in nervous system tissue-specific genes (Neuronal activity, Figure 3), highlighting once more the ability of the method in isolating genes with tissue-specific expression that generally evolve faster than ubiquitously expressed genes.
All other methods succeeded in isolating VLPs and are suitable for quantitative sample comparison if one considers their limitations and pitfalls (discussed above).
The contribution of cell transplantation to cardiac function was examined by functional assessment (see Methods) in freshly isolated Sk-34 cell-transplanted and non-cell-transplanted nude rat MI models.
The retained method consists in isolating image descriptors which are based on image shape parameters such as length and width as well as an angle.
The ABC method assisted in isolating costs of the RUSF component from costs of the overarching FA program, enabling an analysis of incremental costs of the RUSF component.
Biochemical methods employed labeling of macromolecules in isolated hamster treacheas during brief (less than 4 hr) incubation in vitro.
To determine the direct effect of insulin on NPY neurons, we used a method of the [Ca2+]i imaging in isolated single neurons followed by immunocytochemical identification of NPY neurons [ 14, 19].
The model has been simplified by using the Helmholtz decomposition technique and the resulting equations have been solved by using separation of variable method to obtain the secular equations in isolated mathematical conditions for the plates with stress-free or rigidly fixed, thermally insulated and isothermal boundary surfaces.
These methods have succeeded in isolating whole genomes from abundant organisms in some communities, but they are specific to the communities for which they have been devised and often require prior knowledge of the community's composition (Iverson et al. 2012).
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