Lacking of analytical methods for determination of Flutamide and its metabolites inspired us for development of selective, sensitive, and accurate methods for quantitation of Flutamide, Flu-1, and Flu-3.
Clearly, methods for quantitation of gene expression levels at the single cell level are needed to clarify this question.
The strategy made it possible to improve sensitivity and handle small sample volumes, compatible with the stable isotopic label methods for quantitation used in glycoproteomics [ 38].
But hopefully, MRM-based methods for quantitation of cancer-related protein biomarkers will soon be approved by the US FDA [ 42], moving this technique one step closer to clinical application [ 44].
Equivalent responses were observed with both IRP and SID methods for quantitation of six site-specific phosphorylations in the epidermal growth factor receptor (EGFR) in epidermal growth factor-stimulated A431 cells.
Pharmacokinetic analysis had yet to be invented, and methods for quantitation of drugs in serum or plasma were in their infancy, but the spectrophotometric assays that were conducted still provided valuable information.
