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None of the non-chemical methods for parasite control, i.e. nutrition, vaccines, parasite resistant breeds, is sufficiently effective without anthelmintic support and thus do not offer a practical option.
Rapid and accurate methods for parasite detection and monitoring parasite load in VL and PKDL lesion tissues would greatly enhance the clinical management of the disease.
Thus, precise and reliable methods for parasite genotyping or phenotyping during vaccine production and its field application are required.
The gold standard methods for parasite detection and drug susceptibility testing are considered to be microscopic examination of stained blood smears or a radiometric assay measuring the incorporation of [H]-hypoxanthine by the parasite.
The elevated levels of serum antibodies that are generated during the course of infection can provide a sound basis for the development of sensitive and specific serodiagnostic methods for parasite detection.
Similar(55)
At present, PCR is the most sensitive and specific method for parasite detection in malaria-endemic areas.
This paper clearly advances in a very significant way our understanding of heme biosynthesis in the malaria parasite and it provides a provocative potential new method for parasite treatment.
Low budget, rapid methods for monitoring parasite phenotypes are, therefore, crucial, and they could provide logistically important information concerning the potential PZQ resistance status of natural parasite populations and help answer some of the questions posed about drug resistance in human infections.
PCR -based methods for detecting parasites are highly sensitive and have the added advantage that they may be performed on dry specimens without the need for cold-storage [ 22, 23].
Traditional genotyping methods for malarial parasites rely on the size polymorphism of genes encoding surface antigens with variable number of tandem repeats, such as msp2 in P. falciparum, msp3α in P. vivax, and msp1 and csp in both species.
The need for new methods of parasite control is fully recognized by the industry, authorities and society.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com