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All subsequent procedures for parasite isolation and purification were performed in a cold room (4°C) and on ice.
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Examples are the indication for the test: studying symptomatic patients or asymptomatic subjects; preservation of faecal material; and the diagnostic procedures used for parasite testing (PCR or TFT).
A variety of procedures for the diagnosis of parasite stages in coprological samples have been developed, some showing a broad range of application while others are rather specialized methods used only for certain types of parasites.
The procedure for deriving adaptation scores for parasite alleles is the same as of that used for hosts.
The main goal in this research was to determine the beneficial effect of incorporating biological procedures in parasite control programs for equids in zoological parks.
Several PCR-based diagnosis procedures for the identification of these parasites have been developed [ 13- 16].
Procedures for digestion, blotting, and hybridization of parasite genomic DNA with specific pfelo1 and bsd probes are described in detail in the Supporting Information experimental section.
Paradigms for parasite conservation.
This has important implications for parasite survival.
Cholesterol esters were synthesized by the parasite itself and served as energy source for parasite differentiation.
It is best to use two distinct decks to facilitate separation of the host and parasite populations (e.g. blue-backed decks for host, red-backed for parasite).
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