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Exact(4)

These methods for directed evolution differ in the size and characteristics of the gene repertoire, in the way of linking genotype and phenotype, and in the selection approach.

Surface wettability modifications of biocompatible materials and wettability patterning are attractive methods for directed biological cells immobilization for tissue engineering, drug delivery, gene transfer, etc. Hydroxyapatite is known as an implantable biomimetic material and a substrate for effective adhesion of biological cells of various origins.

However, recently developed methods for directed mutagenesis may enable a modified form of TGC in regions that lack P-elements.

(iii) More recently, methods for directed cardiac differentiation starting from FL-free monolayer hESC-derived colonies in defined media were developed.

Similar(56)

Four different methods for directing the incident light onto the particles are examined and the optimum design discussed.

We describe two PCR-based methods for directing point mutations into the yeast genome such that the final product contains no other disruptions.

Here, the first semi-rational method for directed enzyme evolution to enhance methanol tolerance by targeting high B-factor residues for iterative saturation mutagenesis (ISM) is reported.

We combined ideas from CD tagging and directed gene targeting to develop a method for directed fluorescent labeling of endogenous proteins in human cells.

The throughput and suitability of this method for directed evolution is demonstrated by enrichment experiments that recover hits from a sea of 10-fold as many alternative droplets.

The results demonstrated that in situ error-prone PPCP is an efficient random mutagenesis method for directed evolution of genes and proteins.

Many methods used for directed evolution of enzymes were reviewed by Lutz and Patrick.

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