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Here, the first semi-rational method for directed enzyme evolution to enhance methanol tolerance by targeting high B-factor residues for iterative saturation mutagenesis (ISM) is reported.
We report here the development of a random mutagenesis method for directed evolution of Streptomyces lividans acetyl xylan esterase (AxeA), which we previously showed is able to deacetylate chitinous substrate, in order to obtain chitooligosaccharides with well-defined structural properties.
We combined ideas from CD tagging and directed gene targeting to develop a method for directed fluorescent labeling of endogenous proteins in human cells.
The results demonstrated that in situ error-prone PPCP is an efficient random mutagenesis method for directed evolution of genes and proteins.
The throughput and suitability of this method for directed evolution is demonstrated by enrichment experiments that recover hits from a sea of 10-fold as many alternative droplets.
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Categorizing patients with knee pain problems based on pathoanatomical sources has not proved to be the most effective method for directing physical therapy interventions.
Transgenic approaches are therefore an attractive method for directing accumulation of nutritionally balanced, or industrially valuable protein to these large storage organs.
We report here an "intelligent," data-dependent acquisition (DDA) method for directing real-time tandem MS events (MIDA).
This community standard can facilitate the comparisons necessary for formulating relevant biological hypotheses and determining the most appropriate dataset or method for directing further experiments.
Make sure that at the very least, it has the ability to update real-time, and also contains a method for directing traffic to your own website.
These methods for directed evolution differ in the size and characteristics of the gene repertoire, in the way of linking genotype and phenotype, and in the selection approach.
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CEO of Professional Science Editing for Scientists @ prosciediting.com