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All studies were performed using S2R+ cultured cells and standard methods for culture.
Amat, unpublished data; see also [28], [45]), and their ploidy using morphometric methods (for culture conditions and other details see [45], [46]).
Quantitative methods for culture assessment were used at all centers.
Samples were processed by TDH Laboratory Services according to standard methods for culture isolation of mycobacteria (11 ).
Three samples obtained on separate mornings within 1 week were processed by using standard methods for culture isolation of mycobacteria (11 ).
Standard methods for culture of yeast strains and integrative transformation, mating, sporulation and tetrad analysis was performed as previously described [ 63].
Similar(51)
Methods for culturing algae can utilize anthropogenic waste resources including wastewater nutrients and CO2 from fossil fuel combustion.
The development of methods for culturing these cells without using mouse embryonic fibroblasts (MEFs) as a feeder layer will enable more reproducible culture conditions and reduce the risk of xenogenic contaminants, thus increasing the potential clinical applications of differentiated hPSCs.
The use of appropriate cell substrates and methods for culturing these cells for production of vaccines represents a critical factor affecting the maximum viral yield, genetic stability, immunogenicity, and ultimately vaccine safety and economic feasibility.
Most methods for cultures were as described previously.
Graf et al. 's (1992) methods for culturing the flies were followed with some modifications.
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