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Methods for culturing algae can utilize anthropogenic waste resources including wastewater nutrients and CO2 from fossil fuel combustion.
The development of methods for culturing these cells without using mouse embryonic fibroblasts (MEFs) as a feeder layer will enable more reproducible culture conditions and reduce the risk of xenogenic contaminants, thus increasing the potential clinical applications of differentiated hPSCs.
The use of appropriate cell substrates and methods for culturing these cells for production of vaccines represents a critical factor affecting the maximum viral yield, genetic stability, immunogenicity, and ultimately vaccine safety and economic feasibility.
Graf et al. 's (1992) methods for culturing the flies were followed with some modifications.
Furthermore, complicated methods for culturing these bacteria hamper the accurate identification of these pathogens [ 6].
The methods for culturing all the hPSCs provided by the Loring lab are listed in Supplementary information, Table S2.
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Our protocol may be a simple, reliable, and alternative method for culturing MSCs from mouse bone marrow tissues.
This method for culturing islet spheroids could potentially help overcome the cell shortages that have limited clinical applications and could possibly be developed into a bioartificial pancreas.
Here, we present a novel method for culturing kidneys in low volumes of medium that offers more organotypic development compared to conventional methods.
In this study we have adapted a method for culturing cells on biologically relevant "soft" substrates using ECM-conjugated polyacrylamide (PA) gels that can span the stiffness range of 100 Pa–150,000 Pa.
The method for culturing D. pulex was the same as previously described [ 27].
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