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Cell-dependent methods, such as cellular quenching and extraction protocols, have been developed, but no universal methodology for sample pretreatment has been developed yet.
To calculate the sample size, the WHO recommended methodology for sample size calculation for trachoma surveys [16] was used.
Detailed methodology for sample preparation and instrumentation can be found elsewhere [ 69, 70].
We sought to standardize methodology for sample storage, processing and scaling, to recover optimal fcDNA quantities and quality for downstream whole-genome analysis.
Despite this literature the clinical utility of RT PCR for TH mRNA remains unclear, reflecting the small number of patients studied, absence of quality control across studies, lack of uniform methodology for sample collection, processing and inconsistency in reporting (Riley et al, 2003).
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a Sample 1. b Sample 2. c Sample 3 Fig. 4 Methodology for sampling.
Generally speaking, they call on FSIS, EPA and FDA to "1) expand the substances they test for, 2) improve their methodology for sampling hazardous residues, 3) determine more efficient ways of approving newer methods of testing for drug residues and 4) collaborate to set tolerances for additional residues".
Both surveys used the standard DHS methodology for sampling and questionnaire construction, with some minor changes between the 2 surveys.
This, and the fact that GenoTube swabs preserve DNA eliminating the need for refrigeration, makes it a practical, yet reliable methodology for sampling pigs with Brachyspira-associated diarrhea.
In summary, we have described a widely applicable methodology for sampling sequence diversity through phylogenomically guided DNA synthesis coupled to high-throughput NIMS screening to rapidly determine proteins with desirable biochemical properties.
Standardised operating methodologies for sample collection and qPCR testing are provided to aid in the international regulatory diagnosis of M. mackini and serve as a useful platform for the future development of multiplexed or alternate mikrocytid species detection.
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