Since there are no prior methodologies for sampling tweets, we were unable to perform a formal sample size calculation.
Cell-dependent methods, such as cellular quenching and extraction protocols, have been developed, but no universal methodology for sample pretreatment has been developed yet.
To calculate the sample size, the WHO recommended methodology for sample size calculation for trachoma surveys [16] was used.
Detailed methodology for sample preparation and instrumentation can be found elsewhere [ 69, 70].
We sought to standardize methodology for sample storage, processing and scaling, to recover optimal fcDNA quantities and quality for downstream whole-genome analysis.
Despite this literature the clinical utility of RT PCR for TH mRNA remains unclear, reflecting the small number of patients studied, absence of quality control across studies, lack of uniform methodology for sample collection, processing and inconsistency in reporting (Riley et al, 2003).
To this end, we will summarize the main methodologies used for sample preparation and possible methods for the separation, detection, identification, and quantification of proteins/peptides.
The variety of methodologies used for sample preparation, analytical techniques, duration of exposure and post-exposure monitoring may account for these discrepancies.
A minority of studies have not demonstrated an association and this may be attributed to significant differences in the methodologies employed for sample selection, immunostaining techniques, vessel counting and statistical analysis, although a number of biological differences may account for the discrepancy.
