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Each set of experiments was repeated three times, and the relative quantification method with efficiency corrected calculation model (Souaze et al. 1996) was used to evaluate quantitative variations.
Relative expression levels of the genes were calculated in relation to Gapdh expression (Applied Biosystems) using the ΔCt method with efficiency values measured in a pilot experiment for each expression assay.
Relative expression was determined via the 2-ΔΔCT method with efficiency correction [ 50].
For all used primers, amplification efficiencies were determined and mean normalized expression ratios, using HPRT as the reference gene, were calculated using the ΔΔcT method with efficiency correction.
The relative expression of the genes was calculated by the relative quantification method with efficiency correction, using the LightCycler Software 4.1 (Roche).
The amount of gene of interest relative to akuA and h2A mRNA was quantified using the delta delta CT method with efficiency [ 89].
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Moreover, the group reports a faster and very efficient separation method with 98% efficiency with < 1 ppm metallic contaminant levels.
Relative expression levels were calculated using the Pfaffl method [52], with efficiency correction for each primer set, using REST software [52].
Simulations and experimental results show the efficacy of the proposed method with computational efficiency in indoor environments.
Here, we developed a facile phase-transfer method with high efficiency to prepare stable suspensions of graphene oxide in organic solvents.
Three-dimensional numerical simulations were performed for laminar flow of wavy fin-and-tube heat exchangers by using body-fitted coordinates (BFC) method with fin efficiency effect accounted.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com