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Hence, each metabolite concentration was calculated in six different ways; three calculations using the pFID method with calibration data obtained with an underloaded, properly loaded, and overloaded surface coil, and three corresponding concentrations using the phantom replacement method.
External standard method with calibration curve was used for quantification.
In all three centres, serum creatinine was analysed using a Jaffe method with calibration aligned to IDMS.
Relative expression was determined using the comparative cycle threshold (ΔΔCt) method with calibration using samples with the highest levels of RNA.
The clinical biochemistry departments confirmed that, during the study period, eGFR was calculated using the simplified Modification of Diet in Renal Disease equation and creatinine assays conducted using standardised Jaffe's assay method, with calibration traceable to a standard reference material (isotope dilution-mass spectrometry) [ 26- 28].
Plasma edoxaban concentrations were measured by a validated liquid chromatography tandem mass spectrometry (LC-MS/MS) method with calibration ranges of 0.764 to 382 ng/mL (Advion BioServices, Ithaca, NY) for all studies except for the digoxin study, which had a lower limit of quantitation of 1 ng/mL.
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Subsequent studies showed that the phenolic contents of green Brazilian propolis were higher than the flavonoid contents [21] so the total phenol content became a standard test, usually determined by the Folin-Ciocalteau method [22] with calibration curves using phenol [9] or gallic acid [11] as standards.
The DSC was previously calibrated using "Check ʌexo In or ʌexo Zinc" method with Indium/Zinc calibration pans.
PAEE was assessed using the flex HR method with individual calibration of the HR energy expenditure (EE) relationship using indirect calorimetry [ 33] during rest and submaximal exercise as previously described [ 34, 35].
Each pressure sensor was calibrated using associated software (I-Scan 6.10, Tekscan, Inc., South Boston, MA) and a two-point calibration method with low and high calibration point forces of 20 and 600 N, respectively, with a software sensitivity setting of 5, to encompass the expected forces and corresponding pressure readings based on pilot testing.
The linearity and LOD of the method was measured with calibration graphs, also showing SIM spectra and mass measurement data of the protonated species.
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method with correction
method with benchmark
method with standard
method with silicon
method with legislation
method with scenario
method with line
method with sparseness
method with consideration
method with replacement
method with sample
method with efficiency
method with parameter
method with convergence
method with sensitivity
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