Sentence examples for method using monoclonal from inspiring English sources

Immunohistochemical analysis was performed with the avidin-biotin complex method using monoclonal antibodies for glial fibrillary acidic protein, cytokeratin, alpha-internexin, neurofilament proteins (70 kd, 168 kd, and 200 kd), desmin, vimentin, CD34, Ki-67, epithelial membrane antigen, and S-100 protein.

The smears were prepared by means of the immunocytochemical method using monoclonal antibodies for the determination of the oestrogen receptors (ER).

It was concluded that with the new IRMA method using monoclonal antibodies, the diagnostic detectability of serum TA-4 is enhanced in SCC of all organs.

In addition to histopathology, the paraffin-embedded sections were also subjected to immunostaining for CD3 by the standard avidin – biotin method using monoclonal anti-CD3 antibody (PS1, Novocastra, Newcastle-upon-Tyne, UK) as the primary antibody.

The ELISA method, using monoclonal and/or polyclonal antibodies (MAbs and PAbs, respectively) raised against various cellulase enzymes, has been successfully used to quantify target enzymes both in culture filtrates and commercial enzyme preparations [ 19].

The presence of apoptosis was selectively depicted by the Apostain method using monoclonal antibody to single-strand DNA (F7-26 from calf thymus ssDNA, AbCys SA, Paris, France) after DNA denaturation by heating in the presence of formamide, as described by Blaise et al. and Akchiche et al. for tissue sections and cultured cells, respectively.

Briefly, the method uses monoclonal mouse antidog-IgG which recognizes fox antibodies bound to the antigen on the plate, and rabbit antimouse Ig labelled with horse-radish peroxidise [ 4].

Leukemia cell analysis was performed by standard immunofluorescence methods using monoclonal antibodies directed against cMPO, CD33, CD13, CD117, CD9, CD64, CD11b, HLA-DR, CD2, CD19, CD15, CD71, CD34, and CD56.

An indirect intracellular staining (ICS) method was standardized using monoclonal antibodies to different domains of human Factor VIII protein.

A two-step pretargeting method using bispecific monoclonal antibodies [bsmAb] has been developed.

The authors described an indirect intracellular staining method using various monoclonal antibodies to different domain of human factor VIII protein.